cGPS® TI technology

Overexpress a transgene and generate isogenic & stable cell lines with cGPS® TI kits

Cellectis bioresearch offers cGPS® Targeted Integration (TI) kits to generate stable & isogenic cell clones (moncolonal or polyclonal populations), by overexpression a transgene of interest in optimized cGPS® cell lines (CHO-K1 and HEK-293 available).

What is a typical cGPS® TI kit?

Our cGPS® TI Kits comprise all necessary reagents for optimal targeted transgene integration in engineered cGPS® cell lines:

  • cGPS® cell line (CHO-K1 and HEK-293 available)
  • Nuclease
  • Integration Matrices

See all of our available cGPS® TI kits by clicking here.
Please note that each individual component can be purchased separately.

How does a cGPS® TI kit work?

cGPS® TI strategy

  • cGPS® cell line (CHO-K1 and HEK-293 available)

The engineered cGPS® cell line contains a “Landing Pad” (bearing a natural nuclease recognition site) stably integrated in a single copy, to support a highly efficient targeted integration two-step selection process.

  • Nuclease

The nuclease recognizes and cleaves a unique recognition site within the Landing Pad of an engineered cGPS® cell line.

  • Integration Matrix

A plasmid that contains a Multiple Cloning Site (MCS) to clone your gene of interest downstream of a promoter. The transgene expression cassette is flanked by left and right homology arms to drive homologous recombination for targeted transgene integration at the nuclease recognition site. The cGPS® TI kits also include a positive control plasmid.

Please download our cGPS® TI kit sample protocol for a more in depth step by step protocol.
See all of our available cGPS® TI kits by clicking here.

Benefits

Guaranteed targeted integration

Fast

Robust and reliable data

Precise targeted integration to obtain your custom cell line

Increased cell line development productivity with less screening efforts due to high integration frequency (up to 98% efficiency)

Fast and effortless clone selection

As little as 20 days for monoclonal selection (polyclonal selection also available)

 

 

Controlled expression levels

Guaranteed stability of your cell clones without continuous addition of antibiotics (no drug interference)

True isogeny

One transgene copy integrated at one known expression site for comparable and reproducible results

Long compound testing campaigns

Highly stable expression over time (up to 22 weeks)

Not looking for an off the shelf product anymore?
If you would like to take advantage of our in house expertise and resources, let us overexpress a transgene in your cell line of choice based on the cGPS® TI technology.
For more information, please visit our Targeted Integration services page or contact us to discuss your project in more details.

 

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