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VizuCELL
VizuCELL for live cell imaging
VizuCELL cell lines offer an exceptional tool to study temporal and spatial dynamic processes in living cells in order to get critical insights into morphological changes and physiological events in real time. VizuCELL cell lines are appropriate for cell biology, as well as proteomics and genomics.
Benefits:
Generate relevant results |
Ready-to-go imaging |
Easy visualization |
Endogenous level of protein expression:Get information closer to real life models because you are not interfering with cell biology |
Simplified experimental set-up:No input of chemicals, no purification, tagging or introduction of labeled proteins |
Fluorescence tagged cytoskeleton of relevant cell lines:Observe cell migration, morphology and physiological events in real time |
Fluorescence:Suitable for non-invasive long-term monitoring |
Ready to use cell lines:Increased productivity for faster and cost effective workflow |
VizuCELL are generated by utilizing our proprietary TALEN™ technology, to modify cell components with a fluorescent protein (FP) tag by taking advantage of the natural repair mechanism homologues recombination, resulting in endogenous expression of fluorescent fusion protein easily observable by live cell imaging.
Our TALEN™ technology made it possible to modify for the first time HeLa cells through precise gene tagging.
Confocal microscopy analysis of β-actin of VizuCELL U-2 OS hsACTB-GFP – (A) Nuclei, (B) ACTB-GFP, (C) Phalloidin-Rhodamine, (D) Merged picture of A, B and C.
NOW AVAILABLE:
VizuCELL cell lines with fluorescence tagged tubulin and/ or actin genes expressed under control of the endogenous promoter.
Select your cell lines of interest in the table below:
Product name |
Fluorescent tag |
Tagged endogenous protein |
Cat. No. |
|
GFP |
β-actin |
B1-11101013 |
|
|
GFP |
β-actin |
B3-11101017 |
|
| GFP | β-actin | B5-111010 | |
|
RFP |
β-actin |
B1-11102002 |
|
|
RFP |
β-actin |
B3-111020 |
|
|
BFP |
β-actin |
B1-111030B1 |
|
|
GFP |
tubulin α-1β chain |
B1-14101001 |
|
|
GFP |
tubulin α-1β chain |
B3-141010E1 |
|
|
GFP/RFP |
β-actin and tubulin α-1β chain |
B1-11142110 |
|
|
BFP |
histone (H2B type 1-J) |
B1-131030 |
|
|
GFP |
histone (H2B type 1-J) |
B1-131010 |
|
|
GFP/BFP |
tubulin α-1β chain and histone (H2B type 1-J) |
B1-141313 |
U-2 OS is a human osteosarcoma cell line. HeLa is a human cervix carcinoma cell line.
For additional information about the origin of these cell lines, please see product datasheet from ATCC.
See videos of VizuCELL U-2 OS-TUBA1B GFP (left) and VizuCELL U-2 OS-ACTB GFP (right) cells division: