CanCELL HCT-116-RPS6KB1 (-/-)

CanCELL HCT-116-RPS6KB1 (-/-) is a human colon adenocarcinoma cell line with a biallelic knock-out of the RPS6KB1 gene.

CanCELL cell lines are generated by making use of our proprietary TALEN™ technology.

The RPS6KB1 TALEN™ binds precisely its recognition site within the RPS6KB1 gene, inducing a DSB at this site. This DSB is repaired by nonhomologous end joining (NHEJ). Due to the error-prone nature of NHEJ, a proportion of DSBs will be misrepaired by the addition and/or deletion of nucleotides. These frame shift mutations within the genomic sequence occur at the site of the DSB, resulting in functional disruption of a gene’s activity and therefore achieving gene knock-out.

Benefits

• Comparable results
  True isogeny: All knock-out mutants are derived from the same parental cell line
• Convenient and flexible
  Suitable for in vitro and in vivo studies: Allows to keep track of your results – for increased productivity
• Reliable cell model
  STR analyzed: Authentication and certification of parental and gene edited cell lines