cGPS® Targeted Integration is a breakthrough in stable isogenic cell line generation: easy- and ready-to-use, saves time, effort and money!

With cGPS® Targeted Integration kits, sequence-specific nuclease-driven targeted integration can easily be achieved, it requires only the presence of a nuclease recognition site within the targeted cell genome. Although a natural nuclease recognition site is not present in mammalian cells (since the used nucleases are originating single-celled organisms), we propose two ways to carry out sequence-specific targeted integration in higher eukaryotic cells:

1. An engineered cell line is carrying the recognition site of a natural meganuclease as a single copy. This is the principle of our cGPS® line of Targeted Integration kits;
    

GOI: Gene Of Interest

2. An engineered nuclease targets a native, endogenous genomic locus (e.g. a safe harbor). This is the principle of our cGPS® Custom line of Targeted Integration kits.
    

GOI: Gene Of Interest

The key benefits of cGPS® sequence-specific nuclease-driven targeted integration are:

• Easy and ready to use:
  • cGPS® TI kits come with an optimized user manual, which allow an easy set-up of you experiment;
• Fast and time-saving:
• Targeted cell population can be recovered in as little as 2 weeks, isolated clones in 3 to 4, due to the use of selection agents
• Only little clone screening is needed (use of selection agents)
• High clone homogeneity is obtained thanks to very efficient targeted integration protocols (95%+ targeted integration)
  • cGPS® TI products are immediately retargetable cell platforms. There is no prerequisite to first establish such a retargetable platform. Isogenic cell line generation is therefore very fast.
• Effortless, trusted results;
• Trusted results saving time and money:
  • Guaranteed and predictable transgene expression: integration is performed in a single selected transcriptionally active genomic site;
  • Robust data comparison: the generated clones are isogenic;
  • Highly stable expression: for at least 20 weeks, even in the absence of selection agents;
  • Reproducible, highly efficient integration of a single transgene copy at a unique genomic site.