VizuCELL

VizuCELL for live cell imaging

VizuCELL cell lines offer an exceptional tool to study temporal and spatial dynamic processes in living cells in order to get critical insights into morphological changes and physiological events in real time. VizuCELL cell lines are appropriate for cell biology, as well as proteomics and genomics.

Benefits:

Generate relevant results

Ready-to-go imaging

Easy visualization

Endogenous level of protein expression:

Get information closer to real life models because you are not interfering with cell biology

Simplified experimental set-up:

No input of chemicals, no purification, tagging or introduction of labeled proteins

Fluorescence tagged cytoskeleton of relevant cell lines (Hela, U-2 OS):

Observe cell migration, morphology and physiological events in real time

Fluorescence:

Suitable for non-invasive long-term monitoring

Ready to use cell lines:

Increased productivity for faster and cost effective workflow

 

VizuCELL are generated by utilizing our proprietary TALEN™ technology, to modify cell components with a fluorescent protein (FP) tag by taking advantage of the natural repair mechanism homologous recombination, resulting in endogenous expression of fluorescent fusion protein easily observable by live cell imaging.

Our TALEN™ technology made it possible to modify for the first time HeLa cells through precise gene tagging.

 
Confocal microscopy analysis of β-actin of VizuCELL U-2 OS hsACTB-GFP – (A) Nuclei, (B) ACTB-GFP, (C) Phalloidin-Rhodamine, (D) Merged picture of A, B and C.

 

NOW AVAILABLE:

VizuCELL HeLa and U-2 OS cell lines with fluorescence tagged tubulin and/or actin genes expressed under control of the endogenous promoter.

Select your cell lines of interest in the table below:

VizuCELL

FLUORESCENT TAG

GFP

RFP

 BFP GFP/RFP

GFP/BFP

TAGGED CELL COMPONENT

beta-actin

 U-2 OS HeLa U-2 OS HeLa U-2 OS  

 

histone (H2B type 1-J)

 U-2 OS       U-2 OS    

tubulin alpha-1B chain

 U-2 OS HeLa

 

 

  

 

 

beta-actin and tubulin alpha-1B chain

 

 

 

 

  

U-2 OS

  

tubulin alpha-1B chain and histone (H2B type 1-J)

             U-2 OS

U-2 OS is a human osteosarcoma cell line. HeLa is a human cervix carcinoma cell line.

For additional information about the origin of these cell lines, please see product datasheet from ATCC.

 

See videos of VizuCELL U-2 OS-TUBA1B GFP (left) and VizuCELL U-2 OS-ACTB GFP (right) cells division:

 

Copyright 2012 Cellectis
Design & Development by EtonDigital