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TALEN™ Sure KO
Highest knock-out success
TALEN™ Sure KO binds its recognition site within the first coding exon, inducing a DNA double-strand break at the recognition site which is repaired by error prone non homologous end-joining (NHEJ).
The introduction of frame shift mutations in the start codon region of a gene avoids the production of partially functional truncated proteins and greatly ensures gene knock-out events.
The very high modularity of TALEN™ engineering guarantees that there is always a TALEN™ Sure KO for any gene knock-out you want to achieve.
TALEN™ Sure KO’s activity is validated at the level of your choice (on episomal or chromosal target sequence). Click here to see the different validate levels.
Find here publications of gene knock-out with TAL Effector Nucleases.
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Results
In-house results
Internal data demonstrate that TALEN™ Sure KO efficiently knock-out a GFP transgene that has been stably integrated as a single copy in the CHO-K1 genome, with efficiencies of up to 87% within 6 days post TALEN™ transfection.
(A) CHO-K1 cell line stably expressing a single copy of the GFP gene (red curve), compared to a GFP negative CHO-K1 control cell line (blue curve) was transiently transfected with GFP specific TALEN™ Sure KO expression vectors; (C) 6 days post transfection, 87% of cells were found GFP negative (red curve); (B) and (D) confocal images corresponding to (A) and (C) respectively.
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Specifications
Shipment:Room TemperatureStorage:-20°C